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背景 多发性骨髓瘤患者采集干细胞和祖细胞中偶尔存在一定困难。作者回顾性分析了235次这种采集的结果。方法 本研究中共计128例多发性骨髓瘤患者(41例女性,87例男性),其中大多数属于IgG(58％)型,IgA(18％)型和轻链型(18％)。全部操作都是依据使用COBE Spectra Apheresis Device时的标准操作方法而进行的。流速为25和75ml/min之间,进行了2×TBV。对于MNC方法,用1ml/min分离为1000g min,采集Hct为2～3％之间。对于AutoPBSC系统,我们将采集容积定为5ml(采集频率>6)。结果 无论诊断为何种类型的骨髓瘤,54％和88％的患者分别需要进行1次和2次干细胞采集,采集次数与疾病型有关。在这两种COBE Spectra之间,我们发现其血小板损失中值(MNC为42％;AutoPBSC为21％)、CD34+产量/kg(MNC为1.7×10E6/kg;AutoPBSC为2.5×10E6/kg)、CD34+
Background There are occasional difficulties in acquiring stem and progenitor cells in patients with multiple myeloma. The author retrospectively analyzed the results of 235 such acquisitions. Methods A total of 128 patients with multiple myeloma (41 women, 87 men) were enrolled in this study. Most of them were of IgG (58%), IgA (18%), and light chain (18%). All operations are based on standard operating methods when using the COBE Spectra Apheresis Device. Flow rates between 25 and 75 ml/min were performed with 2 x TBV. For the MNC method, it was separated into 1000 g min at 1 ml/min, and the Hct was between 2 and 3%. For the AutoPBSC system, we set the acquisition volume to 5 ml (acquisition frequency >6). Results Regardless of the type of myeloma diagnosed, 54% and 88% of patients required one and two stem cell collections, respectively, and the number of collections was related to the disease type. Between these two COBE Spectras, we found a median platelet loss (MNC of 42%; AutoPBSC of 21%), CD34+ production/kg (MNC of 1.7 x 10E6/kg, AutoPBSC of 2.5 x 10E6/kg), CD34+