目的:研究三叶因子3、基质细胞衍生因子-1(SDF-1)及其受体CXCR4在甲状腺乳头状癌(PTC),中的表达,探讨三者在乳头状癌发生、发展和转归中的作用及相关性。方法:应用免疫组织化学法(SP法)检测92例PTC及癌旁组织中TFF3、SDF-1及CXCR4的表达,采用图像分析软件系统对免疫组织化学结果进行半定量分析。结果:①TFF3蛋白表达于癌细胞胞质,癌旁滤泡上皮细胞TFF3阴性或弱阳性,TFF3阳性率92.39%,其中临床Ⅲ~Ⅳ期强阳性率为71.19%(42/59),Ⅰ~Ⅱ期强阳性率为33.33%(11/33,P<0.01);有淋巴结转移者明显高于无淋巴结转移者(100.00%vs 86.27%,P<0.05)。TFF3的AOD值在PTC内高于癌旁、有淋巴结转移者高于无淋巴结转移者、临床Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期(P<0.05或P<0.01)。②SDF-1表达于胞质,转移淋巴结和癌旁细胞为弱阳性或阴性。SDF-1阳性率和AOD值在PTC内高于癌旁、有淋巴结转移者高于无淋巴结转移者、临床Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期、45岁以上高于45岁以下者(P<0.05或P<0.01);CXCR4主要表达于胞质,少数表达于胞核,癌旁组织中呈弱阳性或阴性,PTC中CXCR4表达的阳性率和AOD值与SDF-1相似:癌内高于癌旁、与临床分级、淋巴结转移和年龄有关(P<0.05或P<0.01)。③PTC中,TFF3与SDF-1蛋白、SDF-1与CXCR4蛋白表达水平呈正相关(r=0.971,P<0.01)。结论:TFF3、SDF-1、CXCR4在PTC中的高表达与癌的发生、发展有关,对判断PTC的恶性程度和病情进展有重要价值。 Objective: To investigate the expression of trefoil factor 3, stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 in papillary thyroid carcinoma (PTC) In the role and relevance. Methods: The expression of TFF3, SDF-1 and CXCR4 in PTC and paracancer tissues was detected by immunohistochemistry (SP method). The immunohistochemistry results were semi-quantitatively analyzed by image analysis software system. Results: (1) The expression of TFF3 was negative or weakly positive in the cytoplasm of cancer cells, and the positive rate of TFF3 was 92.39%. The positive rates of TFF3 in stage Ⅲ ~ Ⅳ were 71.19% (42/59), Ⅰ ~ Ⅱ The positive rate was 33.33% (11/33, P <0.01). The rate of lymph node metastasis was significantly higher than that without lymph node metastasis (100.00% vs 86.27%, P <0.05). The AOD value of TFF3 was higher in PTC than that in adjacent cancers, with lymph node metastasis higher than those without lymph node metastasis. The clinical stage Ⅲ ~ Ⅳ was higher than that in stage Ⅰ ~ Ⅱ (P <0.05 or P <0.01). ②SDF-1 expression in the cytoplasm, lymph node metastasis and para-cancerous cells as weakly positive or negative. The positive rate of SDF-1 and the value of AOD in PTC were higher than that in adjacent cancers, those with lymph node metastasis were higher than those without lymph node metastasis, those in stage Ⅲ-Ⅳ were higher than those in stage Ⅰ-Ⅱ, <0.05 or P <0.01). CXCR4 mainly expressed in the cytoplasm, a small number expressed in the nucleus, paracancerous tissue was weakly positive or negative. The positive rate and AOD value of CXCR4 expression in PTC were similar to that of SDF-1 It was associated with clinical grade, lymph node metastasis and age (P <0.05 or P <0.01). (3) There was a positive correlation between TFF3 and SDF-1 protein, SDF-1 and CXCR4 protein expression in PTC (r = 0.971, P <0.01). Conclusion: The high expression of TFF3, SDF-1 and CXCR4 in PTC is associated with the occurrence and development of cancer, which is of great value in judging the malignancy and progression of PTC.