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不同抗病基因的挖掘是作物持久抗性遗传改良的基础。本研究利用2份抗黑腐病(Xanthamonas campestris pv.campestris)萝卜(Raphanus sativus L.)材料(KB10Q-22、KB10Q-24)和1份感病材料(KB10Q-33)构建了2个F2群体,采用苗期剪叶+喷雾法接种黑腐病菌Xcc8004进行抗病性鉴定。应用P1、P2、F1、F24个世代的数量性状主基因+多基因混合遗传分析方法,研究了萝卜2个不同抗源抗黑腐病的遗传规律,结果表明2份材料的遗传规律不同。以KB10Q-22为母本的F1植株表现为抗病,其遗传模型为E_0模型,即2对加性-显性-上位性主基因+加性-显性-上位性多基因模型;而以KB10Q-24为母本的F1植株表现为感病,其遗传模型为D_0模型,即1对加性-显性主基因+加性-显性-上位性多基因模型。两群体主基因遗传率分别为87.73%和55.64%,抗性遗传以主基因为主。
The excavation of different resistance genes is the basis for the genetic improvement of persistent resistance in crops. In this study, two F2 populations were constructed using two materials of Xanthomonas campestris pv. Campestris (Raphanus sativus L.) (KB10Q-22, KB10Q-24) and one copy of susceptible material (KB10Q-33) , The use of seedling leaf + spray inoculation black rot pathogen Xcc8004 for disease resistance identification. The genetic law of two different resistance to black rot in radish was studied using quantitative trait gene + polygene mixed genetic analysis in P1, P2, F1 and F24 generations. The results showed that the genetic law of the two materials was different. The F1 plants with KB10Q-22 as the female parent showed resistance to disease. The genetic model was E_0 model, that is, two pairs of additive-dominance-epistatic major genes + additive-dominance-epistatic polygene models. The F1 plant of KB10Q-24 was susceptible to the disease. The genetic model was D_0 model, which was a pair of additive-dominant dominant gene plus additive-dominating-epistatic polygene model. The heritabilities of the two groups of major genes were 87.73% and 55.64%, respectively. The major genes of resistance were inherited.