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目的探讨脂蛋白酯酶(lipoprotein lipase,LPL)基因HindⅢ酶切位点多态性在塔吉克族健康人群中的分布及其在不同民族间的分布差异。方法应用聚合酶链反应(PCR)技术检测91例塔吉克族(男性36人,女性55人)LPL基因型并计算出其基因型频率及等位基因频率,并通过查阅文献[10-17]结合统计学软件进行了不同种族间的基因型分布差异比较。结果塔吉克族91例正常人群中H+H+,H+H-,H-H-基因型频率分别为62.64%,31.87%,5.49%,H+,H-等位基因频率分别为78.57%,21.43%。两组男女人群在基因型频率和等位基因频率上均无显著的统计学差异(P=0.652,P=0.187)。而与美国,法国,英国,韩国,印度等人群间比较,基因型和等位基因频率均存在极显著和显著差异(P<0.01,P<0.05),与广西和吉林人群比较差异无显著(P>0.05)。结论新疆塔吉克族人群中存在LPL基因HindⅢ酶切位点多态性在同族男女间无显著性差异,可在不同种族间存在着较大的差异,这种差异可能是导致某些疾病在不同种族间的发现率和临床表现存在显著不同的因素之一。
Objective To investigate the distribution of Hind Ⅲ restriction site polymorphism of lipoprotein lipase (LPL) gene in Tajik healthy people and its distribution among different ethnic groups. Methods The genotypes of LPL in 91 Tajiks (36 males and 55 females) were detected by polymerase chain reaction (PCR) technique and their genotype frequency and allele frequency were calculated. Based on the literature [10-17] Statistical software carried out a comparison of genotypic distribution differences among different races. Results The frequencies of H +, H +, H- and H-H- genotypes in 91 normal population of Tajikistan were 62.64%, 31.87% and 5.49%, respectively. The frequencies of H + and H- alleles were 78.57% and 21.43%, respectively. There was no significant statistical difference in genotype frequency and allele frequency between the two groups of men and women (P = 0.652, P = 0.187). The frequencies of genotypes and alleles were significantly and significantly different from those in the United States, France, Britain, South Korea and India (P <0.01, P <0.05), but not significantly different from those in Guangxi and Jilin P> 0.05). Conclusion There is no significant difference in HPL Ⅲ polymorphism of LPL gene between ethnic groups in Tajik population in Xinjiang. There may be significant differences among different races, which may lead to some diseases in different races One of the factors that significantly different the detection rate and clinical manifestations between.