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目的探讨茶多酚在脂多糖诱导的人支气管上皮细胞损伤中的保护作用及其机制。方法培养人支气管上皮细胞16HBE,给予不同浓度的脂多糖(1,25,50,100μg/ml)和茶多酚(100,200,400,800g/ml)作用。MTT检测细胞的生长,流式细胞术检测细胞凋亡,western blot检测细胞核内和总NFκB的蛋白表达,ELISA检测IL-6,IL-13和TNF-α的含量,试剂盒检测MDA,SOD和LDH的水平。结果与对照组相比,LPS组的细胞生长显著降低;细胞凋亡显著升高;IL-6,IL-13和TNF-α的含量升高;核内和NFκB总蛋白上升;MDA和LDH含量增加,SOD的活性降低,而茶多酚处理抑制以上LPS诱导的16HBE损伤。结论茶多酚可以通过抑制氧化应激缓解脂多糖诱导的人支气管上皮细胞损伤。
Objective To investigate the protective effect of tea polyphenols on lipopolysaccharide-induced human bronchial epithelial cell injury and its mechanism. Methods Cultured human bronchial epithelial cells 16HBE were given different concentrations of lipopolysaccharide (1,25,50,100μg / ml) and tea polyphenols (100,200,400,800 g / ml) role. The expression of IL-6, IL-13 and TNF-α were detected by MTT assay. The apoptosis of cells was detected by flow cytometry. The expression of IL-6, IL-13 and TNF- LDH level. Results Compared with the control group, the cell growth of LPS group was significantly decreased; the apoptosis of LPS group was significantly increased; the levels of IL-6, IL-13 and TNF-α were increased; the content of nuclear and NFκB in total protein and MDA and LDH Increased, SOD activity decreased, while the tea polyphenols treatment inhibited above LPS-induced 16HBE damage. Conclusion Tea polyphenols can alleviate lipopolysaccharide-induced human bronchial epithelial cell injury by inhibiting oxidative stress.