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目的:阪崎肠杆菌(Enterobacter sakazakii)是肠杆菌科肠杆菌属一种革兰阴性菌,是一种条件致病菌,可通过污染婴幼儿配方食品导致严重的婴儿(特别是新生儿)的脑膜炎、坏死性小肠结肠炎和菌血症。对其进行分型研究对阪崎肠杆菌所致疾病的流行病学调查、跟踪和阻断传播途径具有重要的意义。方法:本研究选择7个管家基因atpD、fusA、glnS、gltB、gyrB、infB和pps作为目的基因,对分离自石家庄市售配方米粉、奶粉、婴幼儿食品和环境中分离到的19株阪崎肠杆菌和1株标准株ATCC51329进行了MLST分型检测。结果:20株阪崎肠杆菌可分为12个序列型,分辨率达0.9263,发现9个新ST型。结论:MLST作为阪崎肠杆菌的分型方法,结果准确,分辨率高,对研究该菌的进化趋势和溯源具有重要意义。
Purpose: Enterobacter sakazakii is an Enterobacter family of Enterobacter, a Gram-negative bacterium that is a conditionally pathogenic bacterium that causes severe infants (especially newborns) by contaminating infant formula Meningitis, necrotizing enterocolitis and bacteremia. It is of great importance to conduct epidemiological investigation on the diseases caused by Enterobacter sakazakii, and to track and block the transmission of this disease. Methods: In this study, seven housekeeping genes, atpD, fusA, glnS, gltB, gyrB, infB and pps, were selected as target genes. 19 Sakazaki isolated from commercial rice flour, milk powder, infant food and environment Enterobacter and a standard strain ATCC51329 were MLST type detection. Results: 20 strains of Enterobacter sakazakii were divided into 12 sequences with a resolution of 0.9263 and found 9 new ST types. Conclusion: MLST, as a typing method of Enterobacter sakazakii, has accurate results and high resolution, which is of great significance for studying the evolution trend and traceability of this strain.