论文部分内容阅读
作者对新近诊断的45例MDS患者(RA22例,RAEB23例)作了研究,具体方法为,采集这些患者周围血中的单个核细胞,在α-MEM(含15%胎牛血清及0.4%琼脂)中对洗涤过的细胞进行检定(5×10~5细胞/ml培养物)。培养物的初始渗透压为220mosmol/kg;无外源性集落刺激活性。在5%CO_2条件下,用有一定湿度的保温箱孵育14天,然后计数集落(≥50个细胞/1克隆)。结果证实,诊断时不能检出周围血CFU-GM(PB-CFU-GM)的14例患者,生存期(中位数188天)明显短于PB-CFU-GM能生长的31例患者(中位数>1000天)(P<0.01)。而且,PB-CFU-GM能否检出与RA/RAEB亚型无关。能检出PB-CFU-GM
The authors examined 45 recently diagnosed MDS patients (RA 22, RAEB 23) by collecting mononuclear cells from peripheral blood of these patients and comparing them to α-MEM (containing 15% fetal bovine serum and 0.4% agar The washed cells are assayed (5 x 10 ~ 5 cells / ml culture). The initial osmolarity of the culture was 220 mosmol / kg; no extrinsic colony stimulating activity. Incubate for 14 days in a humidified incubator under 5% CO 2 and count colonies (≥50 cells / 1 clones). The results confirmed that 14 patients who were unable to detect peripheral blood CFU-GM (PB-CFU-GM) at diagnosis had a significantly longer survival (median 188 days) than 31 patients with PB-CFU-GM Digits> 1000 days) (P <0.01). Moreover, whether PB-CFU-GM can be detected is independent of RA / RAEB subtypes. PB-CFU-GM can be detected