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目的:对紫红獐牙菜4个提取部位的降血糖作用进行筛选,并探查其降血糖活性的作用机制是否影响胰岛素的分泌、体重。方法:采用渗漉法提取紫红獐牙菜的乙醇提取物,并将提取物依次萃取分离出乙醚部位、乙酸乙酯部位、正丁醇部位及水溶性4个部位。通过将此4个部位对由STZ诱导的2型糖尿病小鼠进行抗高血糖作用的药理活性研究,根据实验结果对此4个部位进行有效部位筛选,设定高低剂量组从而考察其量效关系。同时观察其对STZ诱导的2型糖尿病小鼠给药物7 d后对小鼠胰岛素抵抗的影响。结果:乙酸乙酯部位及水溶性部位对STZ引起的高血糖小鼠的血糖有较强的抑制作用,而正丁醇及乙醚部位则相对较差。并且各部位对小鼠胰岛素抵抗有十分显著改善。结论:乙酸乙酯及水溶性部位降血糖作用较明显。
OBJECTIVE: To screen the hypoglycemic effects of four extracts from Scutellaria purpurea and investigate whether the mechanism of its hypoglycemic activity affects insulin secretion and body weight. Methods: The ethanol extract of Swertia purpurea was extracted by osmotic method. The extracts were extracted and separated by ether, ethyl acetate, n-butanol and water soluble. The pharmacological activity of these four sites on anti-hyperglycemic effects of STZ-induced type 2 diabetic mice was studied. Based on the experimental results, 4 sites were screened for effective sites, and the doses were set to investigate the dose-effect relationship. . At the same time, the effect of STZ-induced type 2 diabetic mice on insulin resistance after 7 days of drug administration was observed. RESULTS: The ethyl acetate and water-soluble fractions had a strong inhibitory effect on blood glucose in STZ-induced hyperglycemic mice, but n-butanol and ether were relatively poor. And each part has a very significant improvement in the insulin resistance of mice. Conclusion: The hypoglycemic effect of ethyl acetate and water soluble sites is obvious.