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目的探讨竹叶提取物对大鼠急性脑缺血再灌注后脑组织损伤的保护作用。方法取SD大鼠96只,以颈总动脉插入线栓法致大鼠急性脑缺血,缺血60 min后拔线栓造缺血再灌注损伤模型;随机分为假手术组(不插入线栓)、模型组和给药组(缺血前1周、术后,将竹叶提取物按20 mg/kg溶于2 ml生理盐水中腹腔注射)各32只;各组再分为缺血再灌注后6、12、24、48 h 4个亚组,用免疫组化法检测脑组织中的肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、细胞间黏附因子-1(ICAM-1)浓度。结果模型组各个时点脑组织TNF-α、IL-1β、ICAM-1含量较假手术组均明显升高(P均<0.01),TNF-α、IL-1β和ICAM-1分别于12、6、24 h达高峰,以后逐渐降低,48 h时仍高于正常(P均<0.01)。给药组与模型组相应时点比较各指标均明显下降(P<0.01或P<0.05)。结论竹叶提取物可能通过抑制TNF-α、IL-1β、ICAM-1的过度表达,对缺血再灌注后脑组织损伤起保护作用。
Objective To investigate the protective effect of bamboo leaves extract on brain injury after acute cerebral ischemia-reperfusion in rats. Methods Ninety-six SD rats were randomly divided into sham-operation group (no insertion line), sham-operation group The model group and the administration group (one week before and after the operation, the bamboo leaves extract 20 mg / kg dissolved in 2 ml normal saline intraperitoneal injection) each 32; each group is further divided into ischemia At 4, 6, 12, 24, 48 h after reperfusion, the expression of TNF-α, IL-1β, Adhesion factor-1 (ICAM-1) concentration. Results The levels of TNF-α, IL-1β and ICAM-1 in brain tissue in model group were significantly higher than those in sham operation group at each time point (P <0.01) Peaked at 6 and 24 h, then gradually decreased and remained higher than normal at 48 h (all P <0.01). Compared with the model group, the indexes in the administration group and the model group were significantly decreased (P <0.01 or P <0.05). Conclusion Bamboo leaves extract may protect the brain tissue from injury after ischemia-reperfusion by inhibiting the overexpression of TNF-α, IL-1β and ICAM-1.