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目的:运用实时定量PCR检测趋化因子受体CXCR4基因在人乳腺癌组织中的表达及其与肿瘤恶性程度之间的关系。方法:SYBR Green实时定量PCR方法定量检测CXCR4 mRNA在63例乳腺癌(TNM分期:Ⅰ期9例,ⅡA期25例,ⅡB期13例,ⅢA期16例)和20例正常乳腺组织中的表达;采用Kruskal-Wallis检验、Mann-Whit-ney检验等分析CXCR4 mRNA表达在不同临床参数间的表达差异。结果:乳腺癌组织CXCR4 mRNA表达水平(1.22±0.80)高于正常乳腺组织(0.65±0.59),P=0.001。CXCR4 mRNA在乳腺癌中表达上调与淋巴结转移数目(>3个)、HER-2表达情况(+++)密切相关,P值分别为0.013和0.031。但CXCR4 mRNA在不同年龄、绝经情况、组织学分级、病理类型、肿瘤大小以及其他免疫组化指标(ER、PR、p53和Ki-67)组间的差异均无统计学意义,P>0.05。结论:趋化因子受体CXCR4表达可作为预测乳腺癌转移的生物学指标,有望成为乳腺癌治疗的新靶点。
Objective: To detect the expression of chemokine receptor CXCR4 gene in human breast cancer tissues by real-time quantitative polymerase chain reaction and its relationship with the degree of tumor malignancy. METHODS: The expression of CXCR4 mRNA in 63 cases of breast cancer (TNM stage: 9 cases, stage ⅡA 25 cases, stage ⅡB 13 cases, stage ⅢA 16 cases) and normal breast tissue was detected by SYBR Green real-time quantitative polymerase chain reaction Kruskal-Wallis test and Mann-Whitney test were used to analyze the expression of CXCR4 mRNA in different clinical parameters. Results: The expression level of CXCR4 mRNA in breast cancer tissues (1.22 ± 0.80) was higher than that in normal breast tissues (0.65 ± 0.59), P = 0.001. The upregulation of CXCR4 mRNA in breast cancer was closely related to the number of lymph node metastasis (> 3) and the expression of HER-2 (+++), P values were 0.013 and 0.031, respectively. However, there was no significant difference in the expression of CXCR4 mRNA between different age groups, menopause, histological grade, pathological type, tumor size and other immunohistochemical parameters (ER, PR, p53 and Ki-67), P> 0.05. Conclusion: The chemokine receptor CXCR4 expression can be used as a biological indicator to predict the metastasis of breast cancer and is expected to become a new target for the treatment of breast cancer.