Objective:To monitor the levels of aspartate transaminase (AST) and alanine transaminase (ALT) enzymes and testosterone hormone in months and seasons of Iraq.Methods: In this experiment, 20 (2.0-3.5 years old) Iraqi Awassi rams were used which were housed in semi opened shade. Blood samples (2-3 mL) were collected once a week for over period 12 mo. AST and ALT activities were measured by using colorimetric method, and testesterone analysis was performed with an automatic analyzer.Results: The activities of both AST and ALT enzymes were increased significantly (P<0.05) during summer, on monthly basis, June showed the significantly highest value (P<0.05). The present study revealed that testosterone in autumn and summer was recorded significantly highest (P<0.05) in comparison with other seasons and on monthly basis the significant (P<0.05) highest level of testosterone was found between September and August months.Conclusions: AST and ALT enzymes are the highest during summer, and on monthly basis they are the highest in June, while testosterone level is recorded highest in autumn season, and on monthly basis November shows the highest value.