论文部分内容阅读
目的观测家蝇幼虫抗菌肽与美洲大蠊若虫抗菌肽对肿瘤细胞109(人食管癌细胞株)、K562(人髓样白血病细胞株)的作用。方法通过针刺感染大肠杆菌诱导这两种昆虫产生抗菌肽,再经研磨、离心、层析等步骤提取抗菌肽,然后作用于109和K562肿瘤细胞,用流式细胞仪检测有效杀伤率。结果家蝇幼虫抗菌肽对109的有效杀伤率M1为91．0%(对照50．9%),对K562的有效杀伤率M1为95．5%(对照71．3%)；而美洲大蠊若虫抗菌肽对109的有效杀伤率M1为12．6%(对照57．9%),对K562的有效杀伤率M1仅为3．3%(对照68．5%)。家蝇幼虫抗菌肽对两种肿瘤细胞都有较明显的杀伤效果；美洲大蠊若虫抗菌肽对两种肿瘤细胞除了有很小的杀伤力外,更主要的是对其具有促进生长的作用(45．3%,65．2%)。结论两种昆虫抗菌肽产生的抗肿瘤效果不同。
Objective To observe the effects of antibacterial peptides from Musca domestica larvae and nymphs of American cockroach nymphs on tumor cells 109 (human esophageal carcinoma cell line) and K562 (human myeloid leukemia cell line). Methods The two kinds of insects were induced by acupuncture to induce the production of antimicrobial peptides. The antibacterial peptides were extracted by grinding, centrifugation and chromatography. The antimicrobial peptides were then applied to 109 and K562 tumor cells. The killing rate was detected by flow cytometry. The results showed that the effective killing rate of Musca domestica larvae against 109 was 91.0% (control 50.9%) and that of K562 was 95.5% (control 71.3%); while the American cockroach The effective killing rate of nymphs against 109 was M1 was 12.6% (control 57.9%) and the effective killing rate of K562 was only 3.3% (control 68.5%). Musca domestica larvae antibacterial peptides on both tumor cells have a more obvious killing effect; American cockroach nymphs antimicrobial peptides on both tumor cells in addition to a small lethality, the more important is its role in promoting growth ( 45.3%, 65.2%). Conclusion The antitumor effects of two insect antimicrobial peptides are different.