论文部分内容阅读
类泛素的小蛋白修饰(small ubiquitin-like modifier,SUMO)对植物抗病防御起重要作用。本研究利用生物信息学方法,确定了大豆SUMO系统6个相关基因。对细菌性斑点病抗病品种黑农37及感病品种合丰25接种处理后各基因表达变化进行实时定量分析。结果发现:抗病品种中,Gm SUMO2、Gm SCEa在叶中24h表达量最高,Gm SUMO3、Gm SCEb在叶中48h表达量最高,所以Gm SUMO2/3和Gm SCEa/b在大豆叶部抗病过程中起重要作用。Gm SAE2a在茎中48h的表达量高达6倍多,在茎部抗病过程中起主要作用。Gm ESD4e在抗病品种的根和叶中表达量快速下降,起着主要的去SUMO化作用。Gm ESD4a/e在感病品种接菌前期和后期分别在叶和根部相对表达量较高,主导感病品种叶和根部的去SUMO化作用,初步说明大豆SUMO系统与细菌性斑点病抗性相关。
Ubiquitin small-protein modification (small ubiquitin-like modifier, SUMO) play an important role in plant defense against disease. In this study, bioinformatics methods were used to determine 6 related genes of soybean SUMO system. The quantitative analysis of the changes of the gene expression of the disease-resistant variety Heinong 37 and the susceptible variety Hefeng 25 after bacterial inoculation was performed in real time. The results showed that the expression of Gm SUMO2 and Gm SCEa in leaves was the highest at 24 h, and that of Gm SUMO3 and Gm SCEb was highest at 48 h in resistant varieties. Therefore, Gm SUMO2 / 3 and Gm SCEa / Play an important role in the process. The expression level of Gm SAE2a in stem at 48h was more than 6 times, which played a major role in the disease resistance of stems. Gm ESD4e rapidly decreased in roots and leaves of resistant cultivars, and played a major role in desmonization. Gm ESD4a / e had relatively higher expression levels in leaves and roots at the early and late stages of inoculation with susceptible varieties, leading to the de-SUMOylation in the leaves and roots of the susceptible varieties, indicating that the SUMO system in soybean is related to the resistance to bacterial speckles .