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【目的】提高阳离子脂质体介导的肺癌细胞基因转染效率。【方法】采用表达E .coliβ 半乳糖苷酶的 pCMVβ报告基因质粒及组化染色评价阳离子脂质体的转染效率。优化脂质体介导肺癌细胞基因转染效率的条件包括 :脂质体∶DNA电荷比率 ,DNA转染剂量 ,阳离子脂质体的类型。【结果】当阳离子脂质体∶DNA电荷比率 (+ / - )为 3∶1时 ,pCMVβ 的剂量为4μg/孔 (2 4孔板 )时 ,阳离子脂质体DOSPER介导肺癌细胞株SPC A 1转染效率最高 ,达 (11 9± 0 9) %。【结论】优化转染条件 ,脂质体可有效地将外源基因体外导入肺癌细胞。本实验为阳离子脂质体介导肺癌细胞功能性基因的转移及进一步的肺癌基因治疗打下了基础。
【Objective】 To improve the efficiency of cationic liposome-mediated transfection of lung cancer cells. [Methods] The transfection efficiency of cationic liposomes was evaluated using the pCMV beta reporter plasmid expressing E. coli beta galactosidase and histochemical staining. The conditions for optimization of liposome-mediated gene transfection efficiency in lung cancer cells include: liposome:DNA charge ratio, DNA transfection dose, and cationic liposome type. 【Results】 When the cationic liposome:DNA charge ratio (+ / -) was 3:1 and the dose of pCMVβ was 4 μg/well (24-well plate), the cationic liposome-dose-mediated lung cancer cell line SPC A 1 The transfection efficiency was the highest, reaching (11 9 ± 0 9) %. 【Conclusion】Liposome can effectively introduce exogenous genes into lung cancer cells in vitro by optimizing transfection conditions. This experiment laid the foundation for cationic liposome-mediated transfer of functional genes in lung cancer cells and further gene therapy for lung cancer.