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目的:研究肌酸对新生大鼠缺氧心肌葡萄糖摄取的影响,探索改善和调控缺氧心肌能量代谢的因素。方法:将原代培养的新生大鼠心肌细胞随机分为对照组、缺氧组、缺氧联合肌酸组,测定各组心肌细胞2-〔3H〕-脱氧葡萄糖(2-〔3H〕DG)平均摄取率。结果:肌酸浓度为0.5 mmol/L时,心肌细胞对2-〔3H〕DG的摄取率最高。同对照组相比,缺氧组、缺氧联合肌酸组2-〔3H〕DG平均摄取率明显增加。其中缺氧联合肌酸组葡萄糖平均摄取率增加最为明显(P<0.05)。结论:肌酸能够增加缺氧心肌细胞葡萄糖平均摄取率。
Objective: To study the effects of creatine on glucose uptake in hypoxic myocardium of newborn rats and to explore the factors that can improve and regulate energy metabolism in hypoxic myocardium. Methods: Primary cultured neonatal rat cardiomyocytes were randomly divided into control group, hypoxia group and hypoxia combined with creatine group. The levels of 2- [3H] -deoxyglucose (2- [3H] DG) Average intake rate. Results: The uptake rate of 2- [3H] DG in myocardial cells was the highest at 0.5 mmol / L creatine. Compared with the control group, the average uptake rate of 2- [3H] DG in hypoxia group and creatine group increased significantly. Among them, the average glucose uptake ratio in hypoxia combined with creatine group was the most significant (P <0.05). Conclusion: Creatine can increase the average glucose uptake in hypoxic cardiomyocytes.