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目的:研究以1,4-丁二醇二氯甲酸酯为连接剂的聚乙烯亚胺(Polyethyleneimine,PEI)衍生物PEI-Bu对肝癌细胞的转染活性和细胞毒性的影响。方法:以荧光素酶质粒作为报告基因,研究高分子和DNA的复合物在HepG2细胞中的转染活性,研究高分子对Hep G2细胞的毒性。结果:Hep G2细胞转染结果显示我们构建的聚乙烯亚胺衍生物PEI-Bu有比阳性对照PEI25 kDa和Lipofectamine 2000更高的基因表达;细胞毒性结果显示PEI-Bu随着浓度的增加,其毒性显著低于PEI25 kDa。结论:Hep G2细胞实验数据显示PEI-Bu是一种高效、低毒,在肝脏基因治疗领域有相当前景的非病毒载体。
Objective: To investigate the effect of PEI-Bu, a polyethylenimine (PEI) derivative linked with 1,4-butanediol bischloroformate, on the transfection activity and cytotoxicity of hepatoma cells. Methods: The luciferase plasmid was used as a reporter gene to study the transfection activity of the complex of polymer and DNA in HepG2 cells and the toxicity of the polymer to Hep G2 cells. Results: The results of Hep G2 cell transfection showed that PEI-Bu, a polyethyleneimine derivative we constructed, had higher gene expression than the positive control PEI25 kDa and Lipofectamine 2000. Cytotoxicity results showed that PEI-Bu with increasing concentration, Toxicity was significantly lower than PEI25 kDa. Conclusion: The experimental data of Hep G2 cells show that PEI-Bu is a non-viral vector with high efficiency and low toxicity and promising in the field of liver gene therapy.