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目的观察单独和联合使用全反式维甲酸（ＡＴＲＡ）和１，２５－二羟维生素Ｄ３对大肠癌细胞株ＬｏＶｏ碱性磷酸酶（ＡＬＰ）活性的变化的影响。方法用不同剂量的ＡＴＲＡ和１，２５－二羟维生素Ｄ３单独或联合在体外作用于ＬｏＶｏ细胞２、４和６天，用脱氧胆酸钠溶解细胞，动力学方法测定ＡＬＰ活性，紫外吸收法测定蛋白质含量。结果单独或联合使用ＡＴＡＲ和１，２５－二羟维生素Ｄ３均可使ＬｏＶｏ细胞的肠型ＡＬＰ活性逐渐升高，而以联合用药效果更好（Ｐ＜０．０５）。结论联合使用Ａ－ＴＲＡ和１，２５－二羟维生素Ｄ３诱导ＬｏＶｏ细胞比单独用药效果更好，对于大肠癌的诊疗具要的指导意义
Objective To observe the effects of all-trans retinoic acid (ATRA) and 1,25-dihydroxyvitamin D3 on the changes of alkaline phosphatase (ALP) activity in colorectal cancer cell line LoVo. METHODS: Different doses of ATRA and 1,25-dihydroxyvitamin D3 were used alone or in combination to treat LoVo cells in vitro for 2, 4, and 6 days. Cells were lysed with sodium deoxycholate. ALP activity was determined kinetically and determined by ultraviolet absorption. Protein content. Results The use of ATAR and 1,25-dihydroxyvitamin D3 alone or in combination increased the intestinal ALP activity of LoVo cells, and the effect of combination therapy was better (P<0.05). Conclusion The combination of A-TRA and 1,25-dihydroxyvitamin D3 induces LoVo cells to be more effective than medication alone. It has guiding significance for the diagnosis and treatment of colorectal cancer.