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目的:建立胰岛素、C肽实验宣参考值,并与放免法测定的结果进行比较。方法:应用化学发光法和放免法分别测定80例新鲜冰冻血清的胰岛素、C肽值。结果:计算出化学发光法实验室参考值,并与放免法测定的结果相比,胰岛素和C肽的相关系数分别是r1=0.92,r2=0.99。结论:实验室在应用国外生产的试剂盒时,应建立本实验室参考值。同放免法相比,化学发光法有较多的优点。
OBJECTIVE: To establish a reference value for the experimental test of insulin and C-peptide, and to compare with the results of radioimmunoassay. Methods: Chemiluminescence and radioimmunoassay were used to determine the insulin and C-peptide of 80 fresh frozen serum respectively. Results: The reference value of chemiluminescence method was calculated and the correlation coefficients of insulin and C-peptide were r1 = 0.92 and r2 = 0.99, respectively, compared with the results of radioimmunoassay. Conclusion: The laboratory should establish the reference value of this laboratory when applying the kit produced abroad. Chemiluminescence has many advantages over radioimmunoassay.