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目的 分析重庆市高危人群中HIV-1亚型的流行情况。方法 对采自重庆市经性传播和静脉吸毒途径感染的3份HIV-1阳性外周血分离PBMC,扩增env基因C_2-V_3区核酸片段,并进行核苷酸序列分析。结果 在其中一孕妇体内发现一株G亚型HIV-1毒株序列的存在,这是G亚型HIV-1毒株首次在中国被发现,同时有母婴传播的可能;另外两例被证明为E亚型和C亚型HIV-1毒株。结论 这表明HIV在重庆市高危人群中已有流行并且流行情况复杂。
Objective To analyze the prevalence of HIV-1 subtype in high-risk population in Chongqing. Methods PBMCs were isolated from 3 HIV-1 positive peripheral blood collected from sexual transmission and intravenous drug abuse in Chongqing. Nucleic acid fragments of env gene C_2-V_3 region were amplified and sequenced. As a result, one G-type HIV-1 strain was found in one of the pregnant women. This was the first time that the G-type HIV-1 strain was found in China with the possibility of mother-to-child transmission. The other two cases were proved For the E subtype and C subtype HIV-1 strains. Conclusion This suggests that HIV is already prevalent in endemic and high-risk populations in Chongqing and has a complicated epidemic.