以分辨率较高的一米光栅单色仪为分光系统，光电倍增管为检测系统，改进了自行组装的表面等离子体子共振（ＳＰＲ）传感装置，提高了仪器的检测能力约５０倍，对于发展改变波长模式的ＳＰＲ传感器具有重要意义．以对金和抗体均有较强吸附作用的葡萄球菌Ａ蛋白为基底膜，观测了人的Ｂ因子抗体和抗原之间免疫反应的动力学过程，并研究了Ｂ因子的定量测定．结果表明， Ｂ因子抗原的浓度在０·０２～５μｇ／ｍＬ范围内与信号的响应值呈线性关系．该传感器灵敏度高，选择性和重现性均好． A one-meter grating monochromator with high resolution was used as the spectrometer system. The photomultiplier tube was used as the detection system to improve self-assembled surface plasmon resonance (SPR) sensing device, which improved the detection capability of the instrument about 50 times, It is of great importance to develop SPR sensors that change the wavelength mode. The staphylococcal protein A was used as the basement membrane with strong adsorption to both gold and antibody. The kinetics of the immunological reaction between human factor B antibody and antigen was observed. The quantitative determination of factor B was also studied. The results showed that the concentration of factor B antigen in the range of 0.02 ~ 5μg / mL and the signal response was linear. The sensor sensitivity, selectivity and reproducibility are good.