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A Bonner sphere spectrometer (BSS) was developedfoeutrondiagnosticonHL 2ATokamak.It contains eight polyethylene spheres embedded with SP9 3He proportional counter. Before setting up on the Toka-mak experimental hall, a verification experiment was arranged on a 241Am–Be neutron source to test its spec-trometry capability. The neutron response functions were calculated by Monte Carlo code Geant4 to simulate the real measurement environments. By least square method, the neutron spectrum was finally unfolded on log domain from 0.1 eV to 11 MeV. It has a remarkable consistency to the ISO 8529-1 standard 241Am–Be neutron spectrum. This shows that the BSS is effective and reliable for neutron spectrum determination.