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目的 探讨重组人促红细胞生成素(rHuEPO)对心梗大鼠心肌Fas与caspase-9表达的影响.方法 45只SD大鼠,随机分为假手术组、心肌梗死组和rHuEPO治疗组.用Westem blot方法检测各组大鼠梗死区周围心肌中Fas与caspase-9的表达水平,RT-PCR方法检测梗死区周围心肌Fas mRNA与caspase-9 mRNA表达变化.结果 心肌梗死组大鼠心肌梗死区周围心肌Fas与caspase-9蛋白与mRNA的表达水平显著高于假手术组,与心肌梗死组相比,rHuEPO治疗组心肌梗死区周围心肌Fas与caspase-9蛋白与mRNA的表达则明显降低(P＜0.01).结论 rHuEPO下调心梗大鼠心肌梗死区周围心肌Fas与caspase-9的表达.“,”Objective To study the effect of recombinant human erythropoietin (rHuEPO) on the expressions of Fas and caspase-9 in myocardial infarction rats.Methods 45 SD rats were randomly divided into sham group,myocardial infarction group(MI group) and recombinant human erythropoietin group(rHuEPO group).The expressions of Fas and caspase-9 proteins and mRNA in tissue around infarction area were examined by Western blot and RT-PCR methods respectively.Result The expression levels of Fas and caspase-9 protein and mRNA in tissue around infarction area were increased significantly in MI group than in sham group,but decreased significantly in rHuEPO group than in MI group(P＜0.01).Conclusion rHuEPO could down-regulate the expressions of Fas and caspase-9 in myocardial infarction rats.