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目的:观察青蒿琥酯对白血病细胞K562核转录因子κB信号通路的影响。方法:采用Western blot法,检测青蒿琥酯对K562细胞IKKα,IKKβ,IKKγ)表达的影响,采用Western blot法,检测青蒿琥酯对K562细胞caspase 3和PARP cleavage的作用;采用EMSA法检测青蒿琥酯对白血病细胞NF-κB活性的影响。结果:青蒿琥酯能抑制核转录因子κB的表达,青蒿琥酯能够下调IKKα的表达。结论:青蒿琥酯通过下调IKKα的表达而抑制转录因子κB活化。
Objective: To observe the effect of artesunate on K562 nuclear factor kappa B signal pathway in leukemia cells. Methods: The effect of artesunate on the expression of IKKα, IKKβ, IKKγ in K562 cells was detected by Western blot. The effect of artesunate on caspase 3 and PARP cleavage in K562 cells was detected by Western blot. Effect of artesunate on the activity of NF-κB in leukemia cells. RESULTS: Artesunate inhibited the expression of nuclear factor kappa B, and artesunate decreased IKKα expression. Conclusion: Artesunate inhibits the activation of transcription factor κB by down-regulating IKKα expression.