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目的研究人类免疫缺陷病毒(human immunodeficiency virus,HIV)未治疗感染者CD4~+T细胞表面TIGIT受体及其配体CD155表达的情况。方法选取24例未经高效抗逆转录病毒治疗的HIV感染者和20例HIV抗体阴性健康对照(HIVnegative normal control,NC),用流式细胞仪检测受试对象外周血CD4~+T细胞表面TIGIT受体及其相关配体CD155的表达情况。结果未治疗HIV感染者CD4~+T细胞TIGIT受体表达百分数较健康人组表达明显增高(P<0.000 1),并且与CD4~+T细胞绝对计数呈负相关(r=-0.444 1,P=0.029 7)。在未治疗HIV感染者中,CD4~+T细胞计数>350/μl组CD4~+T细胞TIGIT表达百分数明显低于CD4~+T细胞计数≤350/μl组(P=0.029 2);病毒载量>10~5/ml组CD4~+T细胞TIGIT表达百分数明显高于病毒载量≤10~5/ml组(P=0.015 5)。未治疗HIV感染者CD4~+T细胞表面CD155表达百分数与健康人组相比明显增高(P=0.004 2),且与病毒载量呈正相关(r=0.467 7,P=0.021 2),其中病毒载量>10~5/ml组CD4~+T细胞CD155表达百分数明显高于病毒载量≤10~5/ml组(P<0.000 1)。结论未治疗HIV感染者CD4~+T细胞TIGIT受体及其配体CD155表达百分数明显升高,且与CD+4绝对计数及病毒载量存在关联性,可为HIV患者的治疗和预后评估提供重要科学依据。
Objective To study the expression of TIGIT receptor and its ligand CD155 on CD4 ~ + T cells in untreated human immunodeficiency virus (HIV) patients. Methods Twenty-four HIV-negative pregnant women without HIV and 20 HIV negative controls were enrolled in this study. Flow cytometry was used to detect TIGIT Receptor and its related ligand CD155 expression. Results The percentage of TIGIT receptor in CD4 ~ + T cells in untreated HIV-infected patients was significantly higher than that in healthy controls (P <0.000 1), and negatively correlated with absolute CD4 ~ + T cell count (r = -0.4441, P = 0.029 7). The percentage of TIGIT in CD4 ~ + T cells in CD4 ~ + T cells> 350 / μl group was significantly lower than that in CD4 + T cells ≤350 / μl group (P = 0.029 2) The percentage of TIGIT in CD4 ~ + T cells was significantly higher than that in patients with viral load≤10 ~ 5 / ml (P = 0.015 5). The percentage of CD155 on CD4 ~ + T cells in untreated HIV-infected patients was significantly higher than that in healthy controls (P = 0.004 2), and positively correlated with viral load (r = 0.467 7, P = 0.021 2) The percentage of CD155 in CD4 ~ + T cells with load> 10 ~ 5 / ml was significantly higher than that in patients with viral load ≤10 ~ 5 / ml (P <0.0001). Conclusion The percentage of TIGIT receptor and its ligand CD155 in CD4 ~ + T cells in untreated HIV-infected patients was significantly higher than that in non-treated HIV-infected patients, and correlated with absolute CD + 4 count and viral load, which could provide therapeutic and prognostic assessment for HIV patients Important scientific basis.