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为了检测被动型ｎｅｙｍａｎｎｇ肾炎（ＰＨＮ）大鼠细胞免疫状况，我们将抗肾小管刷状缘的抗体腹腔注射大鼠体内而建立了被动Ｈｅｙｍａｎｎ肾炎模型，在ＰＨＮ的最初２８ｄ，用ＭＴＴ比色法检测了７ｄ．１４ｄ、２８ｄ时外周血淋巴细胞诱生ＩＬ－２水平的变化，结果７ｄ时ＩＬ－２水平为２６．５２±１０．７２Ｕ／ｍｌ，与正常对照组３９．８０±９．４０Ｕ／ｍｌ相比显著差异（Ｐ＜０．０１），１４ｄ时ＩＬ－２水平为３０．６８±１１．７９Ｕ／ｍｌ（Ｐ＜Ｏ．０５），２８ｄ时ＩＬ－２水平为３２．７２±８．７２Ｕ／ｍｌ（Ｐ＜０．０５），提示ＰＨＮ大鼠存在免疫功能紊乱。
In order to detect cellular immune status of passive neymanng nephritis (PHN) rats, we established a passive Heymann’s nephritis model by intraperitoneally injecting antibody against the brush border of renal tubule in rats. The first 28 days of PHN were detected by MTT assay 7d. IL-2 levels induced by peripheral blood lymphocytes at 14d, 28d, IL-2 level was 26.52 ± 10.72U / ml on the 7th day, which was significantly lower than that of the control group (39.80 ± 9.40U / ml) (P <0.01). IL-2 level was 30.68 ± 11.79U / ml on the 14th day (P <0.05), and IL-2 level was 32.72 ± 8.72U / ml on the 28th day (P <0.05), suggesting that immune dysfunction exists in PHN rats.