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为了探究饥饿对斑点叉尾血液生理生化指标的影响,于(21±1)℃条件下,在室内水族箱中对斑点叉尾进行为期5周的饥饿试验,分别在饥饿的第0、1、2、3、4周取样并测定其生理生化指标。结果显示,斑点叉尾血液中的红细胞数和红细胞压积分别在饥饿2周和4周内显著下降(P<0.05);而白细胞数和红细胞沉降率分别在饥饿1周与2周内显著增加(P<0.01);血清中血糖对饥饿特别敏感,在饥饿的1～3周内显著下降(P<0.01),此后1周变化不明显(P>0.05);血清中总胆固醇、甘油三酯含量在饥饿3周后均显著降低(P<0.01);饥饿使血清总蛋白和白蛋白浓度呈现先增加后降低的趋势;饥饿对血清氯离子和钙离子浓度没有显著性影响,而碱性磷酸酶活性在饥饿1周显著增加(P<0.05)、谷丙转氨酶活性在饥饿1～2周有非常显著的下降(P<0.01)。总的试验结果显示,饥饿对斑点叉尾大部分的血液生理生化指标有显著性影响。
In order to explore the effect of starvation on the blood physiological and biochemical indexes of Channel Catfish, starvation experiments were carried out in the indoor aquarium for 5 weeks under (21 ± 1) ℃, , 2,3,4 weeks sampling and determination of its physiological and biochemical indicators. The results showed that the number of erythrocytes and hematocrit in the channel catfish decreased significantly at 2 weeks and 4 weeks after starvation (P <0.05), while the numbers of white blood cells and erythrocyte sedimentation rate were significantly different at 1 week and 2 weeks after starvation (P <0.01). Serum glucose was particularly sensitive to starvation and significantly decreased during the first 3 weeks of starvation (P <0.01), but not significantly changed at 1 week (P> 0.05). Serum total cholesterol, triglyceride (P <0.01). The levels of serum total protein and albumin increased first and then decreased with starvation. Starvation had no significant effect on serum chloride and calcium concentrations. However, the content of alkaline phosphatase Activity increased significantly at 1 week (P <0.05), and alanine aminotransferase activity decreased significantly at 1 ~ 2 weeks after starvation (P <0.01). The overall test results show that starvation has a significant effect on most of the blood physiological and biochemical indicators.