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目的观察地锦草总黄酮对衰老小鼠睾丸生精细胞端粒酶表达的影响。方法用D-半乳糖连续ip建立衰老小鼠模型,并ig给予地锦草总黄酮(TFEH);分别采用定量ELISA法、硫代巴比妥酸法及黄嘌呤氧化酶法检测睾丸和脑组织中的端粒酶、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)的活性。结果 TFEH可明显提高衰老小鼠睾丸、脑组织端粒酶的含量和SOD的活性,降低MDA的含量,用药组与衰老模型组比较有明显差异(P<0.05)。结论地锦草总黄酮可明显提高衰老小鼠睾丸、脑组织的抗氧化能力和端粒酶的含量,具有延缓睾丸、脑组织细胞衰老的作用。
Objective To observe the effect of total flavonoids of Erigeron breviocarpa on telomerase expression in spermatogenic cells of testes of senile mice. Methods Aging mice model was established by continuous administration of D-galactose and the total flavonoids (TFEH) of the genus Euphorbiae were given by ig; the contents of testis and brain tissue were measured by quantitative ELISA, thiobarbituric acid and xanthine oxidase In the telomerase, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Results TFEH could significantly increase the contents of telomerase and SOD in testis and brain of aging mice and decrease the content of MDA. Compared with aging model group, TFEH had significant difference (P <0.05). Conclusion The total flavonoids of Sophora flavescens can significantly increase the antioxidant capacity and the content of telomerase in the testis and brain tissue of aging mice, which can delay the senescence of cells in testis and brain.