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目的探讨中国湖南汉族SLE患者与FcγRⅢa—158V/F多态性的相关性。方法SLE患者65例,其中狼疮肾炎患者38例,采用多聚酶链反应(PCR)和限制性片段长度多态性分析法(RFLP)检测FcγRⅢa—158V/F基因型,并与60例正常人进行比较。结果①与对照组相比,SLE患者FcγRⅢa—158F/F纯合子基因型显著增高(OR=2.23,χ2=4.69,P=0.03)。②与对照组相比,狼疮肾炎患者Fc-γRⅢa—158F/F纯合子基因型和FcγRⅢa—158F等位基因频率均显著增高(OR=2.67,χ2=5.36,P=0.02;OR=2.00,χ2=4.91,P=0.03)。结论SLE患者FcγRⅢa—158F/F纯合子基因型显著增高,狼疮肾炎患者FcγRⅢa—158F/F纯合子基因型和FcγRⅢa—158F等位基因频率均显著增高,提示FcγRⅢa多态性与SLE相关,FcγRⅢa—158F等位基因可能是狼疮肾炎的危险因素。
Objective To investigate the association of FcyRⅢa-158V / F polymorphism with SLE in Han nationality in Hunan province of China. Methods Sixty-five patients with SLE and 38 patients with lupus nephritis were enrolled in this study. The genotypes of FcγRⅢa-158V / F were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP), and compared with 60 healthy controls . Results ① Compared with the control group, the genotype of FcyRIIIa-158F / F homozygote in SLE patients was significantly higher (OR = 2.23, χ2 = 4.69, P = 0.03). ② Compared with the control group, the frequency of FcγRⅢa-158F / F homozygous and FcγRⅢa-158F alleles were significantly increased in patients with lupus nephritis (OR = 2.67, χ2 = 5.36, P = 0.02; OR = 2.00, = 4.91, P = 0.03). Conclusion FcyRIIIa-158F / F homozygote genotypes were significantly increased in patients with SLE. The frequencies of FcyRIIIa-158F / F homozygote and FcyRIIIa-158F alleles were significantly increased in patients with lupus nephritis, suggesting that FcyRIIIa polymorphism is associated with SLE, while FcyRIIIa- 158F allele may be a risk factor for lupus nephritis.