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目的:研究单核细胞趋化蛋白-1在不同种植体周围软组织和骨组织中的表达变化。方法:选用8只健康成年杂种狗,拔除双侧下颌第一、二、三前磨牙,3月后植入种植体。采用丝线结扎法建立狗实验性种植体周围炎模型,分别在丝线结扎后1周、2月和6月时处死实验动物,处死动物前记录牙周袋深度(PPD)、菌斑指数(PI)和改良龈沟出血指数(MBI),根据种植体周围炎症情况分健康组、炎症组和种植失败组。取种植体周围软组织用半定量RT-PCR法检测MCP-1 mRNA的表达,取种植体周围骨组织进行免疫组化染色,检测MCP-1的表达情况。用SPSS软件分析不同组别和不同时间点种植体周围软组织和骨组织中MCP-1的表达差异。结果:种植体周围软组织中MCP-1 mRNA的相对表达量在健康组较低(0.11±0.02),显著低于炎症组(0.24±0.05)和种植失败组(0.29±0.04),但炎症组和种植失败组无差别。实验组种植体1周MCP-1 mRNA的相对表达量开始增加,显著高于对照组,1月时最高,但2月和6月时无明显差异。种植体周围骨组织中MCP-1的累积光密度值在健康组(412.36±42.11)低于炎症组(1134.89±56.47)和种植失败组(1345.65±46.23),实验组种植体MCP-1的表达从基点后2月开始增加,对照组无明显变化。结论:MCP-1在种植体周围软组织和骨组织中均有表达,但变化不同步;MCP-1能反映种植体周围软组织的炎症状况,但与种植体周围骨吸收的关系还需进一步研究。
OBJECTIVE: To study the expression changes of monocyte chemoattractant protein-1 in soft tissue and bone tissue around different implants. Methods: Eight healthy adult mongrel dogs were selected. The first, second and third premolar teeth of mandibular lower jaw were removed, and implants were implanted after 3 months. Experimental animal models of peri-implantitis were established by silk ligation. Experimental animals were sacrificed at 1 week, 2 months and 6 months after ligation of the ligaments, respectively. The depths of the periodontal pockets (PPD), plaque index (PI) And improved gingival sulcus bleeding index (MBI), according to the inflammatory conditions around the implant health group, inflammation group and implant failure group. The expression of MCP-1 mRNA was detected by semi-quantitative RT-PCR. The bone around the implant was harvested for immunohistochemical staining to detect the expression of MCP-1. The expression of MCP-1 in soft tissue and bone around implants at different time points and at different time points was analyzed by SPSS software. RESULTS: The relative expression of MCP-1 mRNA in soft tissues around the implant was lower in the healthy group (0.11 ± 0.02) than that in the inflammatory group (0.24 ± 0.05) and the implant failure group (0.29 ± 0.04) There was no difference in the planting failure group. The relative expression of MCP-1 mRNA in the experimental group began to increase at one week, which was significantly higher than that in the control group, the highest at January, but no significant difference between February and June. The cumulative optical density of MCP-1 in the bone tissue around the implant was lower in the healthy group (412.36 ± 42.11) than in the inflammatory group (1134.89 ± 56.47) and in the implant failure group (1345.65 ± 46.23) Increase from the base point after February, no significant change in the control group. CONCLUSIONS: MCP-1 is expressed in soft tissue and bone surrounding dental implants, but the changes are not synchronized. MCP-1 can reflect the inflammatory status of soft tissues surrounding the implants. However, the relationship between MCP-1 and bone resorption remains to be further studied.