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应用ＰＣＲ及ＰＡＧＥ技术研究了Ｄ１９Ｓ２５３基因座的多态性，调查广州地区无关个体，获得广州人群中Ｄ１９Ｓ２５３基因座频率分布，结果显示Ｄ１９Ｓ２５３位点扩增片段分布于２０９－２４１ｂｐ之间，基因型频率分布与Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡吻合良好。分别计算基因座的杂合度（Ｈ）、个人识别能力（ＤＰ）、非父排除率（ＰＥ）和多态信息含量（ＰＩＣ）。并制作位点特异性等位基因梯阶（Ｌｏｃｕｓｓｐｅｃｉｆｉｃａｌｅｌｉｃｌａｄｄｅｒ）作结果比对，使基因判型简便准确，并有利于计算机贮存、管理资料
The polymorphism of D19S253 locus was investigated by PCR and PAGE. The frequency of D19S253 locus in Guangzhou population was investigated. The results showed that the amplified fragment of D19S253 locus was located between 209-241bp. The genotype frequency The distribution is in good agreement with the Hardy-Weinberg equilibrium. Heterozygosity (H), personal identification ability (DP), non-parent exclusion rate (PE) and polymorphism information content (PIC) were calculated respectively. And making site-specific allelic ladder (Locusspecificalelicladder) for the results of the comparison, so that the gene is simple and accurate, and is conducive to computer storage, management information