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目的:观察黄芪总皂甙(AS)及黄芪注射液(AI)对病毒性心肌炎(VMC)小鼠心肌损伤的保护作用及其对心肌肌浆网钙泵的影响。方法:腹腔接种柯萨奇B3亲心肌病毒株建立小鼠VMC模型,分成模型组、AS组、AI组及正常对照组,分别观察各组小鼠死亡率、心肌病理变化、血清心肌肌钙蛋白I(cTnI)含量及心肌肌浆网钙泵(SERCA)活力等指标。结果:模型组死亡率高于对照组(P=00042)、AS组及AI组(P<005);cTnI含量显著高于对照组(P<0001),也高于AS组(P<0025)及AI组(P<005)。AS及AI组的心肌坏死和炎症等病理改变则轻于模型组(P<001)。模型组心肌SERCA活力显著低于对照组(P<0001),AS组(P<001)及AI组(P<005)。结论:AS和AI对VMC小鼠心肌损害具有不同程度的保护作用,AS是黄芪治疗VMC的有效成分;改善VMC小鼠心肌SERCA的活力,可能是黄芪及AS保护感染病毒小鼠心肌免受损害的作用机制之一。
Objective: To observe the protective effect of astragalus saponins (AS) and Astragalus membranaceus (AI) on myocardial injury in mice with viral myocarditis (VMC) and its effect on myocardial sarcoplasmic reticulum calcium pump. Methods: The model of VMC was established by intraperitoneal inoculation of Coxsackievirus B3 pro-cardiomyocyte strain and divided into model group, AS group, AI group and normal control group. The mortality, myocardial pathological changes, serum cardiac troponin I (cTnI) content and myocardial sarcoplasmic reticulum calcium pump (SERCA) activity and other indicators. Results: The mortality of model group was higher than that of control group (P = 00042), AS group and AI group (P <005), cTnI level was significantly higher than that of control group (P < And AI group (P <005). The pathological changes of myocardial necrosis and inflammation in AS and AI group were lighter than those in model group (P <001). SERCA activity in model group was significantly lower than that in control group (P <0.001), AS group (P <001) and AI group (P <005). CONCLUSION: AS and AI have protective effect on myocardium damage of VMC mice to varying degrees, AS is the active ingredient of Astragalus membranaceus for treating VMC. To improve the activity of SERCA in myocardium of VMC mice may be that Astragalus membranaceus and AS protect the myocardium of infected mice from damage One of the mechanisms of action.