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目的：观察携带胶质细胞源性神经营养因子（ｇｌｉａｌｃｅｌｌｉｎｅｄｅｒｉｖｅｄｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ，ＧＤＮＦ）基因的重组腺病毒（ＡｄＣＭＶｇｄｎｆ）对损伤运动神经元的保护作用。方法：采用培养运动神经元的谷氨酸损伤模型，评价ＧＤＮＦ重组腺病毒对损伤运动神经元的影响，指标是运动神经元计数。结果：（１）建立了运动神经元谷氨酸损伤模型：当培养液中加入０．５ｍｍｏｌ·Ｌ－１的谷氨酸，作用２４～４８ｈ后，运动神经元数目较对照组显著减少（Ｐ＜０．０１）；（２）携带ＧＤＮＦ基因的重组腺病毒可显著减轻谷氨酸导致的运动神经元损伤。结论：腺病毒介导的ＧＤＮＦ表达对损伤的运动神经元具有保护作用。
Objective: To observe the protective effect of recombinant adenovirus (AdCMVgdnf) carrying glial cell linederived neurotrophic factor (GDNF) gene on injured motor neurons. Methods: The glutamate injury model of cultured motor neuron was used to evaluate the effect of GDNF recombinant adenovirus on injured motor neuron. The index was motor neuron count. Results: (1) The glutamate injury model of motor neuron was established. When 0.5mmol·L-1 glutamic acid was added into the culture medium, the number of motor neurons decreased significantly after 24-48h (P < 0.01). (2) The recombinant adenovirus carrying GDNF gene can significantly reduce glutamate-induced motor neuron injury. CONCLUSION: Adenovirus-mediated GDNF expression has a protective effect on injured motor neurons.