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目的 观察慢性铅暴露对小鼠大脑皮层神经元磷脂酰肌醇3激酶(PI3Ks)表达的影响.方法 昆明系小鼠出生第1 d通过饮水饲以不同浓度醋酸铅(1.2,2.4,4.8,7.2,9.6 mmoL/L)制备染铅模型,对照组饲以等量自来水;6周后用石墨炉原子吸收光谱法测血铅脑铅浓度;用蛋白印迹(Western blot)法观察各组小鼠大脑皮层神经元PI3Ks的表达状况.结果 慢性染铅组小鼠大脑皮层神经元PI3Ks表达水平呈下降趋势,各染铅组PI3Ks表达与对照组比较,差异有统计学意义,并具有浓度效应关系(P<0.05);PI3Ks表达与血铅浓度呈负相关(r=-0.82,P<0.01).结论 慢性染铅可使大脑皮层神经元细胞PI3Ks表达水平下降;这可能是铅导致学习记忆功能损伤的原因之一.“,”Objective To explore the expression of phosphatidylinositol 3-kinase(PI3Ks) in cortical neuron of mice with chronic lead exposure.Methods Acetic lead(1.2,2.4,4.8,7.2, and 9.6 mmol/L) in drinking water was applied to mice for 6 weeks.At the end of the exposure, blood lead concentration and brain lead concentration in the mice were detemi-ned with graphite furnace atomic absorption spectrometry and the expression of PI3Ks was determined with Western blot-ting.Results Compared with the control group,the expression of PI3Ks in cortical neuron of lead exposure group was de-creased in significant dose-dependent manner (P < 0.05).The expression of PI3Ks in cortical neuron was negatively correla-ted with the concentration of blood lead and brain lead (r = - 0.82,P < 0.01).Conclusion Chronic acetic lead exposure can depress the expression of PI3Ks in mice which is maybe one of the reasons for lead-induced damage of learning and memory.