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目的探讨甲基硒酸(Me Se A)、硒代蛋氨酸(Se Met)和甲基硒代半胱氨酸(Me Se Cys)对宫颈癌He La细胞的增殖、迁移及粘附功能的影响。方法体外培养He La细胞,3μmol/L的Me Se A、Se Met和Me Se Cys处理He La细胞12~72 h,分别采用MTT法、划痕实验和体外基质粘附实验检测He La细胞的增殖、迁移及粘附功能。结果与对照细胞相比,Me Se A处理组He La细胞的增殖速度明显降低(P<0.01)。Me Se A处理组He La细胞在4 h和8 h的迁移抑制率分别为34%(P<0.05)和26%(P<0.05);Se Met处理组He La细胞在4 h和8 h的迁移抑制率分别为18%和13%;Me Se Cys处理组He La细胞在4 h和8 h的迁移抑制率分别为28%(P<0.05)和5%。Me Se A、Se Met和Me Se Cys对He La细胞粘附功能的抑制率分别为36%(P<0.01)、25%和49%(P<0.01)。结论 Me Se A可以更好地抑制He La细胞的增殖和迁移,而Me Se Cys对He La细胞的粘附功能的抑制作用更强。
Objective To investigate the effects of Me Se A, Se Met and Me Se Cys on the proliferation, migration and adhesion of cervical cancer HeLa cells. Methods HeLa cells were cultured in vitro. HeLa cells were treated with 3μmol / L Me Se A, Se Met and Me Se Cys for 12-72 h. MTT assay, scratch assay and in vitro matrix adhesion assay were used to detect He La cells proliferation , Migration and adhesion function. Results Compared with control cells, the proliferation rate of HeLa cells treated with Me Se A was significantly decreased (P <0.01). The inhibitory rates of He La cells migration at 4 h and 8 h in Me Se A group were 34% (P <0.05) and 26% (P <0.05), respectively. Migration inhibition rates were 18% and 13%, respectively. The inhibitory rates of migration of HeLa cells treated with Me Se Cys at 4 h and 8 h were 28% (P <0.05) and 5%, respectively. The inhibitory rates of Me Se A, Se Met and Me Se Cys on He La cell adhesion were 36% (P <0.01), 25% and 49% (P <0.01), respectively. Conclusion Me Se A can inhibit the proliferation and migration of He La cells better, while Me Se Cys can inhibit the adhesion of He La cells more strongly.