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目的 制定并考察小春花药材质量标准,为进一步开发小春花药材提供基础.方法 采用紫外-可见分光光度法建立小春花中总黄酮的含量测定方法 ;采用HPLC同时测定小春花药材中槲皮素和山奈酚的含量.结果 建立小春花总黄酮的测定方法 ,槲皮素为在30.23～181.40μg·mL-1呈良好线性关系,R2=0.9984;确定药材中槲皮素、山奈酚的含量测定方法 ,槲皮素在2.64～21.12 μg·mL-1呈良好的线性关系,R2=0.999 8,山奈酚在2.00～16.00 μg·mL-1呈良好的线性关系, R2=0.999 9;小春花总黄酮以槲皮素计不得＜0.2‰,山奈酚计不得＜0.6‰.结论 本研究对小春花药材进行质量控制,提升并建立了新的小春花药材质量标准.“,”OBJECTIVE To establish the quality standards for Sceptridium ternatum, and lay the foundation for its further development. METHODS The content of total flavonoids was assayed by UV-Vis spectrophotometry. The contents of quercetin and kaempferol were determined by HPLC. RESULTS There was a good linear relationship between the absorbance values and the quercetin concentration when it was in the range of 30.23-181.40 μg·mL-1(R2=0.998 4). The quercetin and kaempferol were in good linear relationships in the range of 2.64-21.12 μg·mL-1(R2=0.999 8) and 2.00-16.00 μg·mL-1(R2= 0.999 9), respectively. The quercetin/kaempferol content in Sceptridium ternatum should not be less than 0.2％o and 0.6‰, respectively. CONCLUSION The quality control standard for Sceptridium ternatum is modified and a new one is provided to guarantee the quality of raw medical materials.