论文部分内容阅读
目的 :研究羧甲基壳聚糖锌多肽复合材料(carboxymethyl chitosan zinc and peptide,CMC-Zn+-P)对人牙周膜成纤维细胞(human periodontal ligament cells,HPDLCs)的细胞毒性,为材料的安全使用提供依据。方法:体外培养人牙周膜成纤维细胞,并进行形态学及免疫组织化学鉴定。使用含不同浓度材料浸提液的细胞培养液培养细胞,并进行分组,1~4组为实验组,材料浸提液浓度分别为100%、75%、50%和25%,第5组为对照组,不含材料浸提液。采用CCK-8法测定复合材料不同浓度浸提液对细胞增殖活性的影响,通过细胞相对增殖率进行细胞毒性评级。采用SPSS 17.0软件包对数据进行统计学分析。结果 :原代培养的细胞呈成纤维细胞形态,免疫组织化学染色结果显示细胞波形丝蛋白染色阳性,角蛋白染色阴性,属中胚层组织来源。不同浓度材料浸提液对细胞均有促进增殖的作用,相对增殖率均大于100%,细胞毒性分级为0级。结论:CMC-Zn+-P复合材料对体外培养的HPDLCs无细胞毒性,符合国家标准的要求。
Objective: To study the cytotoxicity of carboxymethyl chitosan zinc and peptide (CMC-Zn + -P) on human periodontal ligament cells (HPDLCs) Use to provide basis. Methods: Human periodontal ligament fibroblasts were cultured in vitro and identified by morphology and immunohistochemistry. The cell culture medium containing different concentrations of material leaching solution was cultured and divided into groups. Groups 1 to 4 were experimental groups, the concentrations of material leaching solution were 100%, 75%, 50% and 25%, respectively. The fifth group was Control group, without material extract. CCK-8 method was used to determine the effect of different concentrations of composite extracts on cell proliferation activity. Cytotoxicity was evaluated by cell relative proliferation rate. Data were statistically analyzed using SPSS 17.0 software package. Results: Primary cultured cells showed fibroblast morphology. The results of immunohistochemical staining showed that the cells were positive for silk fibroin staining and negative for keratin staining, which were the source of mesoderm tissue. Different concentrations of material leaching solution on the proliferation of cells have a role, the relative proliferation rate were greater than 100%, cytotoxicity grade 0. Conclusion: CMC-Zn + -P composites have no cytotoxicity to HPDLCs cultured in vitro and meet the requirements of national standards.