An orthogonal experimental design OA_9(3~3) was used to evaluate the effects of vitamins(A, B_2, and B_6) on the growth and digestive ability of the juvenile Chinese soft-shelled turtle, Pelodiscus sinensis(initial weight, 5.9±0.2 g). A total of 135 turtles were divided into 9 groups, which each included 15 individuals. The results revealed that vitamin A(VA) had the strongest impacts on the growth rate and feed utilization among the three vitamins; 35,000 IU kg~(-1) VA had optimal effects on the feeding intake and specific growth rate, and 20,000 IU kg~(-1) VA had optimal effects on protein digestibility and the feed conversion ratio. Vitamin B_2(VB_2) was essential for regulating protein deposition and the energy efficiency for growth of the turtles; 120 mg kg~(-1) VB_2 resulted in increased protein and energy deposition, and 180 mg kg~(-1) VB_2 had greater beneficial effects on the growth rate. Vitamin B_6(VB_6) had important effects on protein and feed efficiency; however, VB_6 at an excessive level(120 mg kg~(-1)) restricted turtle growth. Based on the above growth results, dietary supplementation of VA, VB_2 and VB_6 at levels of 35,000 IU kg~(-1), 180 mg kg~(-1) and 70 mg kg~(-1), respectively, were recommended for the juvenile soft-shelled turtle. An orthogonal experimental design OA_9 (3~3) was used to evaluate the effects of vitamins (A, B_2, and B_6) on the growth and digestive ability of the juvenile Chinese soft-shelled turtle, Pelodiscus sinensis (initial weight, 5.9 ± 0.2 g). A total of 135 turtles were divided into 9 groups, which each included 15 individuals. The results revealed that vitamin A (VA) had the strongest impacts on the growth rate and feed utilization among the three vitamins; 35,000 IU kg ~ ( -1) VA had optimal effects on the feeding intake and specific growth rate, and 20,000 IU kg -1 VA had optimal effects on protein digestibility and the feed conversion ratio. Vitamin B 2 (VB 2) was essential for regulating protein deposition and the energy efficiency for growth of the turtles; 120 mg kg -1 VB 2 resulted in more protein and energy deposition, and 180 mg kg -1 VB_2 had more beneficial effects on the growth rate. Vitamin B_6 (VB_6) had important effects on protein and feed efficiency; however, VB_6 at Based on the above growth results, dietary supplementation of VA, VB_2 and VB_6 at levels of 35,000 IU kg -1, 180 mg kg ~ (-1) 1) and 70 mg kg ~ (-1), respectively, were recommended for the juvenile soft-shelled turtle.