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通过静态吸附与解析实验,比较了7种大孔树脂对杨梅叶原花色素的纯化效果。结果表明,HPD-500型大孔树脂的吸附效果最好。通过单因素实验,确定HPD-500型大孔树脂对杨梅叶原花色素的最佳吸附浓度为7.0mg/mL,吸附平衡时间为3h,吸附量可达到(106.61±7.83)mg/g大孔树脂;解吸液为50%乙醇水溶液,解吸时间为30min,解吸率达到96.67%。经过解吸后的产物,原花色素的含量从原来的26%提升到了45.3%,并除去了原有粗提物中的大部分糖,总糖含量从原来的24.7%下降到了4.4%。为了提高样品和大孔树脂的利用率,采用了静态吸附和动态吸附结合的方法,将固形物含量为11.83%的含有26%原花色素的粗提物溶液540mL与200g HPD-500型大孔树脂在砂芯玻璃层析柱中进行两次吸附解吸,合并两次解吸产物,基本除去了糖,原花色素含量提升至53%,原花色素的得率在80%以上,实现了原花色素的有效纯化。
By means of static adsorption and analytical experiments, seven kinds of macroporous resins were compared to purify the pigment of bayberry leaf proanthocyanidins. The results show that, HPD-500 macroporous resin adsorption best. Through the single factor experiment, the optimum adsorption concentration of HPD-500 macroporous resin on the proanthocyanidin of Bayberry leaf was 7.0mg / mL, the adsorption equilibrium time was 3h, the adsorption capacity could reach (106.61 ± 7.83) mg / g macropore Resin; desorption solution was 50% ethanol solution, desorption time was 30min, desorption rate reached 96.67%. After desorption of the product, the proanthocyanidin content increased from 26% to 45.3%, and removed most of the original crude extract of sugar, the total sugar content decreased from 24.7% to 4.4%. In order to improve the utilization of sample and macroporous resin, a static adsorption and dynamic adsorption combined with the method, the solid content of 11.83% containing 26% proanthocyanidins crude extract solution 540mL and 200g HPD-500-type macropores The resin was adsorbed and desorbed twice in a sand core glass column and the desorbed product was combined twice to remove the sugar. The proanthocyanidin content was increased to 53% and the proanthocyanidin yield was above 80%. The original flower Effective purification of pigments.