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将油菜菌核病菌 (S .sclerotiorum)之菌核作为诱饵埋入油菜土壤中 ,二个月后从菌核表面和内部分离到 5 2株木霉 ,其中黄绿木霉 (T .aureoviride) 2 5株 ,占总数 48 1% ;钩状木霉 (T .hamatum) 17株 ,32 7% ;哈茨木霉 (T .harzianum ) 7株 ,13 3% ;康氏木霉 (T .koningii) 2株 ,4 0 % ,以及拟康氏木霉 (T .pseudokoningii) 1株 ,2 %。将木霉菌株与核盘菌在PDA平板上的玻璃纸条上对峙培养 ,发现木霉菌丝平行于核盘菌菌丝生长 ,并伸出短枝样菌丝或勾状菌丝紧紧附着在核盘菌丝上 ,最终导致核盘菌菌丝断裂和消解。如果两菌直接在PDA平板上双相生长 ,2 4h后两菌落接触 ,在多数情形下 ,在核盘菌一侧会产生褐色、黄褐色宽窄不等的拮抗带 ,拮抗带中的核盘菌菌丝肿胀、断裂和原生质解体。 2周后有 42个木霉菌落覆盖过核盘菌菌落 ,占供试木霉菌株的 84%。而且核盘菌菌落中形成的菌核数量比对照明显减少 ,有些木霉菌株能完全抑制菌核产生。木霉的非挥发性代谢产物对核盘菌菌丝的生长、菌核的数量和大小有明显影响。而挥发性代谢产物仅仅对菌核大小有一定抑制作用。将拮抗作用强的木霉菌株培养物导入土壤后 ,埋入土壤中的菌核多数将发生腐烂 ,即使未腐烂的菌核其产子囊盘萌发能力也大大降低
The sclerotinia sclerotiorum sclerotinia (S. sclerotiorum) sclerotia as a bait buried in rape soil, two months later from the sclerotium surface and internal separation of 52 Trichoderma strains, Trichoderma reesei (T aureoviride) 25 , Accounting for 48.1% of the total; 17 strains of T. hamatum (17.7%), 7 strains of T. harzianum (13.3%), 2 strains of T. koningii 40%, and 1 strain of T. pseudokoningii, 2%. Trichoderma strains and Sclerotinia in the PDA cellophane confrontation culture on the plate and found Trichoderma parallel to the proliferation of Sclerotinia mycelium and stick out mycelium or hook stick mycelium tightly attached to Nuclear disk mycelium, eventually leading to nuclear fungus mycelium break and digestion. If the two bacteria directly on the PDA plate biphasic growth, 2 4h after the two colonies contact, in most cases, Sclerotinia side will produce brown, brown width ranging from antagonistic bands, antagonistic with the sclerotinia Mycelium swelling, rupture and protoplasm disintegration. Two weeks later, 42 Trichoderma colonies covered S. pneumoniae colonies, accounting for 84% of the tested Trichoderma strains. Moreover, the number of sclerotia formed in Sclerotinia sclerotiorum was significantly reduced compared with the control, and some Trichoderma strains could completely inhibit sclerotinia. The non-volatile metabolite of Trichoderma had a significant effect on the growth of sclerotinia mycelium and the number and size of sclerotia. The volatile metabolites only have a certain inhibitory effect on the size of sclerotia. After the antagonistic Trichoderma strain culture was introduced into the soil, most of the sclerotia embedded in the soil would decay, even if the non-decayed sclerotium also greatly reduced its germinal vesicle germination capacity