论文部分内容阅读
基因型是影响发根农杆菌转化大豆的主要因素之一,为筛选转化效率高的大豆基因型,利用不同基因型大豆的下胚轴接种发根农杆菌K599(含GUS基因),待长出毛状根后,根据不同基因型大豆发根诱导率和发根数的差异,选择适宜大豆基因型进行GUS基因的组织化学染色和RT-PCR分析。结果表明,34个大豆基因型发根诱导率和发根数有很大差异,选择发根诱导率大于60%以及发根数大于1.6条的20个大豆基因型毛状根进行GUS基因组织化学染色,其中Y091、Z184、P108、L010等4个大豆基因型的GUS基因转化效率较高,分别为96.2%、91.7%、87.5%、86.4%;进一步对以上4个大豆基因型毛状根进行RT-PCR分析,结果表明,GUS基因均得到了正确转录。表明大豆基因型Y091、Z184、P108、L010是发根农杆菌转化大豆较为理想的受体材料。
Genotypes are one of the major factors affecting Agrobacterium rhizogenes transformation of soybean. In order to screen soybean genotypes with high transformation efficiency, Agrobacterium rhizogenes K599 (including GUS gene) was inoculated on the hypocotyls of different genotypes of soybean to be grown Hairy roots, according to different genotypes of soybean hair root induction rate and the number of hair roots, select appropriate genotypes of GUS gene histochemical staining and RT-PCR analysis. The results showed that 34 soybean genotypes had significant differences in hair root induction rate and hair root number. 20 soybean hairy roots with more than 60% hair root induction rate and more than 1.6 hair root number were selected for GUS genomics The genotypes of Y091, Z184, P108, and L010 were highly efficient for the transformation of GUS genes, which were 96.2%, 91.7%, 87.5% and 86.4%, respectively. Furthermore, the hairy roots of the above four soybean genotypes RT-PCR analysis showed that the GUS gene was correctly transcribed. The results showed that the soybean genotypes Y091, Z184, P108 and L010 were the ideal recipient materials for Agrobacterium rhizogenes transformation of soybean.