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目的 探讨二氧化碳气体对结肠肿瘤细胞转移能力的影响。方法 体外培养人结肠癌CCL - 2 2 8细胞 ,应用二氧化碳或氮气处理不同时间后 ,应用免疫组织化学方法检测细胞中E钙粘蛋白 (E -Cadherin)和血管内皮生长因子 (VEGF)表达的变化。结果 二氧化碳处理 6 0min后CCL - 2 2 8细胞E -Cadherin表达显著降低 ,而VEGF表达显著增加。而氮气处理对上述指标产生相同影响但其作用明显弱于二氧化碳。结论 6 0min以上时间的二氧化碳气腹可以明显增强肿瘤细胞的转移能力 ,而氮气对其影响明显弱于二氧化碳 ,序贯应用二氧化碳、氮气、二氧化碳气腹的方法可改善二氧化碳气腹所致的影响。
Objective To investigate the effect of carbon dioxide gas on the metastasis of colon tumor cells. Methods Human colon cancer CCL - 2 2 8 cells were cultured in vitro. After treated with carbon dioxide or nitrogen for different time, the expression of E - cadherin and vascular endothelial growth factor (VEGF) in the cells were detected by immunohistochemistry . Results After carbon dioxide treatment for 60 min, the expression of E - cadherin in CCL - 2 28 cells was significantly decreased, while the expression of VEGF was significantly increased. Nitrogen treatment has the same effect on the above indicators but its effect is obviously weaker than that of carbon dioxide. Conclusions Carbon dioxide pneumoperitoneum can significantly enhance the metastasis ability of tumor cells over 60 minutes, while the effect of nitrogen on carbon dioxide is obviously weaker than that on carbon dioxide. Sequential application of CO2, N2 and CO2 pneumoperitoneum can improve the effect of CO2 pneumoperitoneum.