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以4—10Gy射线在体损伤的小鼠为模型,应用DNA电泳和流式细胞术等方法证实细胞凋亡是射线损伤在体骨髓造血细胞的途径之一,发现射线诱导的造血细胞凋亡有明显的量效和时效关系。4、6、8和10Gy照射后,细胞凋亡发生率均表现为升高-降低过程,各剂量诱导的凋亡发生率峰值分别出现在照后12、8、4和4h,6和8Gy诱导的凋亡发生率已达最高水平,约为30％,10Gy诱导的凋亡反而要低。上述结果表明,诱导细胞凋亡是射线损伤骨髓造血细胞的一个重要途径,而且凋亡的发生率受照射剂量和照后时间的影响。因此,深入研究射线诱导的细胞凋亡,将有助于揭示射线损伤造血功能的机理。
4-10Gy ray in vivo injury in mice as a model, the application of DNA electrophoresis and flow cytometry and other methods to confirm apoptosis is ray damage in bone marrow cells in one of the ways and found that radiation-induced hematopoietic apoptosis Significant dose-effect and aging relationship. After 4, 6, 8, and 10 Gy irradiation, the apoptotic rates all showed ascending-descending process, and the peak incidence of apoptosis induced by each dose appeared at 12, 8, 4 and 4h, 6 and 8 Gy The incidence of apoptosis has reached the highest level, about 30%, 10Gy induced apoptosis but lower. The above results show that the induction of apoptosis is an important way of radiation injury of bone marrow hematopoietic cells, and the incidence of apoptosis is affected by the dose and time after irradiation. Therefore, in-depth study of radiation-induced apoptosis will help reveal the mechanism of radiation injury hematopoietic function.