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目的 :探讨一氧化氮 (NO)核因子κB(NF κB)信号通路在人幼稚T细胞向 1型辅助性T细胞 (Th1) /2型辅助性T细胞(Th2 )分化过程中的作用。方法 :分离人脐血幼稚T细胞 ,分别加入不同浓度的NO供体硝普钠 (SNP)、NO合成抑制剂左旋精氨酸甲基酯 (NAME)和NF κB抑制剂吡咯二硫氨基甲酸酯(PDTC) ,通过流式细胞术检测细胞内IFN γIL 4的表达 ,了解幼稚T细胞的分化。结果 :在不同浓度的SNP和NAME和PDTC作用下 ,表达IFN γ(即Th1)或IL 4(即Th2 )T细胞的百分率与对照组相比较均无显著差异 (P >0 .0 5)。结论 :NONF κB信号通路对人幼稚T细胞向Th1和Th2细胞的分化均无显著影响。该通路在Th1/Th2型细胞因子表达过程中的调控作用可能主要发生于成熟的Th1和Th2细胞水平
AIM: To investigate the role of nuclear factor-kappa B (NF-κB) signaling in the differentiation of human naive T cells into type 1 T helper (Th1) / type 2 T helper (Th2) cells. Methods: Adventitial naive T cells from human umbilical cord blood were isolated and treated with NO donor sodium nitroprusside (SNP), NO synthesis inhibitor L-arginine methyl ester (NAME) and NF κB inhibitor pyrrolic dithiocarbamate Ester (PDTC) was used to detect the expression of intracellular IFNγIL 4 by flow cytometry to understand the differentiation of naive T cells. Results: The percentages of IFNγ (Th1) or IL4 (Th2) T cells were not significantly different from those of the control group (P> 0.05) under the different concentrations of SNP and NAME and PDTC. Conclusion: NONF κB signaling pathway had no significant effect on the differentiation of human naive T cells into Th1 and Th2 cells. The regulatory role of this pathway in Th1 / Th2 cytokine expression may occur mainly at mature Th1 and Th2 cell levels