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目的建立HPLC法同时测定朝鲜淫羊藿提取物中淫羊藿苷A、淫羊霍苷、朝藿定B、淫羊藿次苷Ⅱ含量的方法。方法色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm),流动相为甲醇-水梯度洗脱[0~25 min,w(甲醇)=55%~70%;25~40 min,w(甲醇)=70%~90%;40~45 min,w(甲醇)=90%~55%;45~60 min,w(甲醇)=55%],流速为0.8 mL.min-1,检测波长为270 nm,柱温为25℃。结果淫羊藿苷A、淫羊霍苷、朝藿定B、淫羊藿次苷Ⅱ的线性关系良好,线性范围分别为16.5~82.5、96.0~480.0、30.6~153.0、1.5~7.5 mg.L-1,平均回收率分别为103.7%、100.9%、102.2%、100.1%,RSD分别为1.4%、2.9%、1.2%、2.5%(n=5)。结论该方法可作为朝鲜淫羊藿提取物的含量测定方法 ,为其质量控制提供参考依据。
OBJECTIVE To establish a HPLC method for the simultaneous determination of Icariin A, Epimedium, Epimedin B and Epimedium Ⅱ in the extract of Epimedium koreanum. Methods The mobile phase consisted of Diamonsil C18 (250 mm × 4.6 mm, 5 μm) with a mobile phase of methanol-water gradient (0-25 min, w (methanol) = 55-70% ) = 70% -90%; 40-45 min, w (methanol) = 90% -55%; 45-60 min, w (methanol) = 55%] at a flow rate of 0.8 mL.min- 270 nm, the column temperature is 25 ℃. Results The linear relationship between icariin A, icariin, epimedin B and epimedium Ⅱ was good with linear ranges of 16.5 ~ 82.5, 96.0 ~ 480.0, 30.6 ~ 153.0 and 1.5 ~ 7.5 mg · L -1, and the average recoveries were 103.7%, 100.9%, 102.2% and 100.1%, respectively. The RSDs were 1.4%, 2.9%, 1.2% and 2.5%, respectively. Conclusion This method can be used as a method for the determination of the extract of Epimedium koreanum and provide a reference for its quality control.