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目的:应用激光房水细胞仪(LFCM)定量分析PVR动物模型中的炎症反应及PDTC对房水闪光的影响。方法:青紫蓝家兔20只随机等分成2组,在视网膜上制造裂孔后,向第1组所有家兔的右眼(A1组)及第2组所有家兔的左眼(A2组)玻璃体腔注射PDTC0.1mL,向第2组所有家兔的右眼(B1)玻璃体腔注射BSS0.1mL。1h后向实验眼A1组注射BSS0.1mL,向实验眼A2组及实验眼B1组玻璃体腔注射5000UIL-1β。分别于术前及第二次注射后4,24h,1,2及4wk进行临床观察及LFCM检查。病理学及免疫组织化学检查分别在术后4,24h,1,2及4wk进行。结果:在术后24h～2wkPDTC能够明显抑制PVR动物模型中的炎症反应。虽然在术后2wk时应用裂隙灯显微镜观察各组炎症反应已完全消退,但应用LFCM发现实验眼B1组炎症反应仍比另2组明显。病理学及免疫组织化学检查表明IL-1β能够活化NF-κB,PDTC能够抑制其活化而没有明显视网膜毒性作用。结论:在玻璃体腔注射IL-1β诱导的PVR动物模型中有炎症反应的参与,PDTC可以明显抑制此炎症反应。LFCM提供了一种新的,灵敏的,客观和非侵入式的方法对PVR动物模型中的炎症反应进行定量分析。
OBJECTIVE: To quantitatively analyze the inflammatory reaction in PVR animal model and the effect of PDTC on the flash of aqueous humor by using laser chamber water cytometry (LFCM). Methods: Twenty rabbits were randomly divided into two groups. After making holes in the retina, the rabbits in group 1 were exposed to the right eye (group A1) and the rabbits in group 2 (group A2) PDTC 0.1 mL was injected into the body cavity and 0.1 mL of BSS was injected into the right eye (B1) vitreous cavity of all rabbits in the second group. After 1h, 0.1 mL BSS was injected into experimental group A1, and 5000 UIL-1β was injected into the vitreous cavity of experimental group A2 and experimental group B1. Clinical observation and LFCM examination were performed before and after 4, 24 hours, 1, 2 and 4 weeks after the second injection respectively. Pathology and immunohistochemistry were performed at 4, 24 h, 1, 2 and 4 wk postoperatively. Results: After 24h ~ 2wk PDTC could significantly inhibit the inflammation in PVR animal model. Although the inflammatory response in each group was completely resolved by slit lamp microscopy 2wk after operation, the inflammatory reaction in group B1 was still more obvious than that in the other two groups by LFCM. Pathological and immunohistochemical examination showed that IL-1β can activate NF-κB, PDTC can inhibit its activation without obvious retinal toxicity. CONCLUSION: Inflammatory response is involved in the intravitreal injection of IL-1β-induced PVR in animal models. PDTC can significantly inhibit this inflammatory response. LFCM provides a new, sensitive, objective and noninvasive method for the quantitative analysis of inflammatory responses in animal models of PVR.