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目的探讨严重脓毒症患儿外周血自然杀伤(natural killer,NK)细胞及各亚群比例的变化。方法以2009年1月—2012年12月收治并临床治愈的严重脓毒症患儿8例为研究对象,所有患儿于诊断严重脓毒症24 h内(急性期)及病情稳定进入恢复期后抽取外周血检测N K细胞及各亚群:CD56+NK细胞、CD16+NK细胞、CD56+16+NK细胞百分率。计量资料采用t检验,P<0.05为差异有统计学意义。结果 NK细胞、CD56+CD16+NK细胞、CD16+NK细胞比例恢复期[(5.70±2.77)%、(4.75±3.23)%、(0.53±0.41)%]均较急性期[(4.44±0.90)%、(3.73±1.21)%、(0.41±0.28)%]明显升高,比较差异均有统计学意义(均P<0.05)。结论严重脓毒症患儿表达CD16+分子的NK细胞数量,恢复期较急性期明显升高。这可能是严重脓毒症患儿能进入恢复期或能治愈的重要原因之一。
Objective To investigate the changes of the proportion of natural killer (NK) cells and their subpopulations in children with severe sepsis. Methods Eight children with severe sepsis who were treated and clinically cured from January 2009 to December 2012 were enrolled in this study. All children were admitted to the convalescence within 24 h after diagnosis of severe sepsis (acute phase) and stable condition Peripheral blood samples were collected for detection of NK cells and their subsets: CD56 + NK cells, CD16 + NK cells, and CD56 + 16 + NK cells. Measurement data using t test, P <0.05 for the difference was statistically significant. Results The recovery rate of NK cells, CD56 + CD16 + NK cells and CD16 + NK cells in acute stage [(4.70 ± 2.77)%, (4.75 ± 3.23)%, (0.53 ± 0.41)%] %, (3.73 ± 1.21)% and (0.41 ± 0.28)%, respectively, which were significantly different between the two groups (all P <0.05). Conclusion The number of CD16 + NK cells in children with severe sepsis is significantly higher than that in acute phase. This may be one of the important reasons that children with severe sepsis can enter the recovery phase or can be cured.