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从明恢63平衡化cDNA文库中获得水稻GS2基因的cDNA克隆,通过基因克隆和农杆菌介导的水稻遗传转化方法将GS2基因导入中花11进行超量表达.对获得的转基因植株进行生长表型和基因表达、生理生化指标测定与分析.结果显示,T1代转基因植株由于GS2的抑制表达出现黄化现象,生长受到限制;叶片叶绿素含量与GS2基因的表达量紧密相关;植株中大多数氮代谢、叶绿素合成和光呼吸途径中相关基因表达量上升;而植株的氮代谢水平下降,包括GS活性、水溶性蛋白和氨基酸含量。
The cDNA clone of rice GS2 gene was obtained from the Minghui63 balanced cDNA library, and the GS2 gene was overexpressed into Zhonghua 11 by gene cloning and Agrobacterium-mediated rice genetic transformation. The results showed that T1 generation transgenic plants showed yellowing due to GS2 inhibition and their growth was restricted. Chlorophyll content in leaves was closely related to GS2 gene expression. Most of the nitrogen Metabolism, chlorophyll synthesis and photorespiratory pathways increased the expression of related genes; while the nitrogen metabolism of plants decreased, including GS activity, water-soluble protein and amino acid content.