论文部分内容阅读
为了解胶质瘤p16基因突变和蛋白丢失与其恶性程度的关系,我们用两种方法分别检测45例脑胶质瘤p16基因第2外显子的突变和蛋白丢失情况,结果报道如下对象和方法1.标本来源和DNA制备 45例脑胶质瘤标本取自我科手术标本。经病理学检查按WHO肿瘤分类标准:12例星形细胞瘤(WHO Ⅱ),18例间变性星形细胞瘤(WHO Ⅲ),15例胶质母细胞瘤(WHO Ⅳ)。每1标本分两份,一份置-70℃冰箱用于p16基因突变检测,一份石腊包埋用于p16蛋白丢失检测。每例取癌旁组织作为对照。DNA提取按常规方法进行。2.PCR引物及反应条件引物由北京医科大学设计合成。反应条件:94℃1分钟;用上、下游引物时62℃
To understand the relationship between glioma p16 gene mutation and protein loss and its malignancy, we detected the mutation and protein loss of exon 2 of 45 glioma p16 genes by two methods. The results were reported as follows 1. Specimens and DNA Preparation 45 cases of glioma specimens taken from our surgical specimens. The pathological examination according to WHO classification of tumors: 12 cases of astrocytoma (WHO Ⅱ), 18 cases of anaplastic astrocytoma (WHO Ⅲ), 15 cases of glioblastoma (WHO Ⅳ). Each sample was divided into two parts, one set at -70 ℃ refrigerator for p16 gene mutation detection, a paraffin embedded for p16 protein loss detection. Each case of paracancerous tissue as a control. DNA extraction is carried out according to a conventional method. 2.PCR primers and reaction conditions primers designed and synthesized by Beijing Medical University. Reaction conditions: 1 min at 94 ° C; 62 ° C with upstream and downstream primers