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目的探讨自分泌运动因子Autotaxin(ATX)在人卵巢癌中的表达及其与卵巢癌彩色血管能量成像(CPA)特征之间的关系。方法术前应用经阴道CPA检查,术后应用半定量逆转录聚合酶链反应(RT-PCR)、Western blot印迹研究8例正常卵巢组织、18例良性卵巢上皮性肿瘤、50例卵巢上皮性癌组织、23例大网膜转移灶中ATX mRNA的表达,以-βactin为内参照。结果正常卵巢、卵巢良性肿瘤、卵巢癌、大网膜转移灶中均有ATX mRNA不同程度表达;但ATX基因表达值癌组织和大网膜转移灶中显著高于良性卵巢肿瘤和正常卵巢组织[分别为(89.31±10.15)%(、88.91±11.05)%(、40.18±16.71)%、(35.29±13.82)%,P<0.001]。Western blot显示ATX蛋白在卵巢癌细胞中明显表达,在55 kD6、0 kD大小的位置可见清楚显色的条带。ATXmRNA在卵巢癌细胞亦可见明显扩增条带。说明卵巢癌细胞中ATX在核酸与蛋白水平均有表达,两者结果一致。高表达ATX卵巢癌与癌细胞转移、癌栓形成、肿瘤的分化程度有关,而与年龄、肿瘤大小等无关。卵巢癌CPAⅢ型病例的ATX表达明显高于Ⅰ型和Ⅱ型病例,实性区少的上皮性癌乳头区ATX表达最高。结论卵巢癌细胞中ATX分子在核酸与蛋白水平均有表达,两者结果一致;CPA为临床鉴别良恶性卵巢肿瘤提供了敏感的血流信息,且CPA血流特性与ATX基因、蛋白表达存在正相关,ATX过度表达与卵巢癌的进展、转移有关。
Objective To investigate the expression of autotaxin (ATX) in human ovarian cancer and its relationship with the features of color vessel energy imaging (CPA) in ovarian cancer. Methods The transvaginal CPA examination was performed before operation. Eight cases of normal ovarian tissue, 18 cases of benign epithelial ovarian tumor and 50 cases of epithelial ovarian cancer were studied by semi-quantitative RT-PCR and Western blotting. Tissues, 23 cases of omental metastasis ATX mRNA expression, -βactin as an internal reference. Results The expression of ATX mRNA in normal ovary, benign ovarian tumor, ovarian cancer and omental metastasis were all significantly different, but ATX gene expression was significantly higher in cancerous tissue and omental metastasis than in benign ovarian tumor and normal ovarian tissue [ (89.31 ± 10.15)% (88.91 ± 11.05)% (40.18 ± 16.71)%, (35.29 ± 13.82)%, P <0.001 respectively. Western blot showed that ATX protein was overexpressed in ovarian cancer cells, and a clearly colored band was visible at the position of 0 kD at 55 kD6. ATXmRNA also showed obvious amplification bands in ovarian cancer cells. It shows that ATX is expressed in both nucleic acid and protein level in ovarian cancer cells. High expression of ATX ovarian cancer and cancer metastasis, tumor thrombus formation, the degree of tumor differentiation, but not with age, tumor size and so on. ATX expression in ovarian cancer CPA Ⅲ cases was significantly higher than that in cases Ⅰ and Ⅱ, and ATX expression was the highest in epithelial cancer papillary area with less real area. Conclusions ATX molecules in ovarian cancer cells are expressed at both nucleic acid and protein levels, and the results are consistent. CPA provides sensitive blood flow information for the differential diagnosis of benign and malignant ovarian tumors, and CPA blood flow characteristics and ATX gene and protein expression are positive Related, ATX overexpression and ovarian cancer progress, metastasis.