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将ＢａｍＨⅠ酶切的人脑髓鞘碱性蛋白（ＭＢＰ）基因ｃＤＮＡ克隆片段和表达载体ｐＧＥＸ－５Ｔ重组后转化大肠杆菌，再筛选、增殖和ＩＰＴＧ诱导。结果显示：阳性克隆１０％ＳＤＳ－ＰＡＧＥ有２条特异区带（４９ｋｄ和３５ｋｄ）；Ｗｅｓｔｅｒｎ印迹杂交证实该区带具ＭＢＰ抗原特异性；蛋白质含量测定、免疫斑点杂交和ＥＬＩＳＡ分析，该膜蛋白表达量占菌体裂解液蛋白质总量６％（５０ｍｇ／Ｌ菌液）。
The cDNA fragment of human MBP gene was digested with BamHI and transformed into Escherichia coli with the expression vector pGEX-5T. The recombinant plasmid was transformed into Escherichia coli and then screened, proliferated and induced by IPTG. The results showed that there were two specific bands (49kd and 35kd) on 10% SDS-PAGE of positive clones. Western blotting confirmed the specificity of MBP antigen in this region; protein content, immunofluorescence and ELISA analysis Amount accounted for 6% of the total bacterial lysate protein (50mg / L bacteria).